The aim of individuals consuming health supplements is to attain a robust state through nutritional regulation.However, some unscrupulous manufacturers, motivated by profit, fraudulently incorporate drugs or unauthorized components with therapeutic effects into the product for instant product performance enhancement.The long-term use of these products may inadvertently inflict harm on human health and fail to promote nutritive healthcare.The illegal inclusion of these substances is prevalent in kidney-tonifying and sexuality-enhancing verona wig products.Developing effective analytical methods to identify these products and screen for illegal added ingredients can effectively prevent such products from reaching and remaining on the market.
A target screening method for the detection and quantification of 90 phosphodiesterase type 5 inhibitors (PDE-5is) in 5 kinds of health products was developed and validated.The type of dietary supplements varied from tablets, capsules, and protein powder to wine and beverages.Sample preparation was completed with a one-step liquid phase extraction.The screening process of 90 PDE-5is was done efficiently within 25 min by ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) using the dynamic multiple reaction monitoring (dMRM) technique.The LODs of 90 PDE-5is were detected at levels ranging from 25 to 85 ng/g or ng/mL.
This novel targeting methodology was olea europaea montra effective and can be applied to routine market supervision.Among 286 batches of samples, 8 batches were found to be positive.Three kinds of PDE-5is were first detected in healthy products.The screening method demonstrated herein will be a promising and powerful tool for rapid screening of PDE-5is.